Category EV L13 The Effect of Pain Relievers Polluting Water on P. caudatum and L.

variegatus

Abstract As people take medicine, not all of the medicine is metabolized by the body,

some of it is excreted. People also commonly dispose unused medications

down the drain. Recent investigations reveal that these medications end

up polluting natural bodies of water and affecting wildlife living in the

water. However, the effects of these medications on wildlife are relatively

unknown. This project was to determine what effects do the pain

relievers that are found in natural bodies of water have on the behavior of

unicellular and multicellular organisms. Paramecium caudatum, and

Lumbriculus variegatus were used as biological indicators and were

exposed to non-prescription naproxen, ibuprofen, aspirin, and

acetaminophen to determine their behavior when pain relievers are

released into the water.

P. caudatum were exposed to pain relievers dissolved in water at various

concentrations. After 48 hours, the swim speed (mm per sec) and number

of direction changes (turns per sec) of P. caudatum were recorded. The

results revealed that as the concentration level increased, there were

more direction changes and slower swim speed.

Lumbriculus variegatus were exposed to pain relievers dissolved in water

at various concentrations. Their heartbeats were recorded after 24, 48

and 72 hours under a microscope. As the concentration increased their

heartbeat increased and many died.

The lowest concentrations that were tested were in the parts per billion.

The results show that even low concentrations of pain relievers in water

can greatly affect the behavior of aquatic organisms.

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